Prof. Dr. rer nat. Franz E. Weber
Extraordinarius: Craniofacial and Oral Biotechnology
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Research Interests
In cranio-maxillofacial and oral surgery the substitution of a mandible to restore resections of tumors for example, still poses a great challenge. To overcome this problem our oral Biotechnology and Bioengineering laboratory works on the development of new osteoconductive bone substitute materials and their combination with osteoinductive bone morphogenetic proteins, and epigenetically active small molecules. Based on our capabilities to design and produce ceramic based scaffolds with additive manufacturing, we try to unravel the essence of osteoconduction as important driving force of bone regeneration. The materials we use and develop range from degradable polylactide based materials, to natural and synthetic hydrogels, like fibrin and polyethylenglycol-(PEG)-based hydrogels, to titanium and ceramic based scaffolds produced in a patient specific shape by additive manufacturing. The delivery of osteoinductive bone morphogenetic proteins, including epigenetically active enhancers of bone morphogenetic proteins and degradable, bioactive guided bone regeneration membranes are other fields of expertise of our laboratory. Moreover, we work on the regeneration of the pulp using natural and synthetic hydrogels and diverse factors with the aim to extend the lifetime of teeth.
Selected publications
Ivica A, Zehnder M, Mateos JM, Ghayor C, and Weber FE (2019) Biomimetic Conditioning of Human Dentin Using Citric Acid.
Int Endod J. 45: 45-50
Ghayor C & Weber FE (2018) Osteoconductive microarchitecture of bone substitutes for bone regeneration revisited.
Front. Physiol. doi: 10.3389/fphys.2018.00960
Ghayor C., Gjoksi B., Dong J., Siegenthaler B., Caflisch A., Weber FE (2017) N,N Dimethylacetamide a drug excipient that acts as bromodomain ligand for osteoporosis treatment.
Scientific Reports Feb 8;7:42108
de Wild M, Zimmermann S, Rüegg J, Schumacher R, Fleischmann T, Ghayor C, Weber FE (2016) Influence of microarchitecture on osteoconduction and mechanics of porous titanium scaffolds generated by selective laser melting
3D printing + Additve manufacturing, 3(3): 143-151
Karfeld-Sulzer L.S., Ghayor C., Siegenthaler B., de Wild M., Leroux J.-C., Weber FE (2015) N-methyl Pyrrolidone/Bone Morphogenetic Protein-2 Double Delivery with In Situ Forming Implants
Journal of Controlled Release. 203C: 181-188.
Sala A, Hänseler P, Ranga A, Lutolf MP, Vörös J, Ehrbar M, Weber FE. (2011). Engineering 3D cell instructive microenvironments by rational assembly of artificial extracellular matrices and cell patterning.
Integr. Biol. 3 (11):1102 – 1111
Lutolf MP, Weber FE, Schmoekel HG, Schense JC, Kohler T, Müller R and JA Hubbell (2003). Repair of bone defects using synthetic mimetics of collagenous extracellular matrices.
Nature Biotechnol. 21: 513-518
Links
Publications in ZORA (Zurich Open Repository and Archive)
ZORA Publication List
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Publications
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Optimizing Filament-Based TCP Scaffold Design for Osteoconduction and Bone Augmentation: Insights from In Vivo Rabbit Models. Journal of Functional Biomaterials, 15(7):174.
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TPMS Microarchitectures for Vertical Bone Augmentation and Osteoconduction: An In Vivo Study. Materials, 17(11):2533.
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Patient-specific beta-tricalcium phosphate scaffold for customized alveolar ridge augmentation: a case report. International Journal of Implant Dentistry, 10(1):21.
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Functionalization of Ceramic Scaffolds with Exosomes from Bone Marrow Mesenchymal Stromal Cells for Bone Tissue Engineering. International Journal of Molecular Sciences, 25(7):3826.
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Enhanced bone regeneration in rat calvarial defects through BMP2 release from engineered poly(ethylene glycol) hydrogels. Scientific Reports, 14(1):4916.
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Three-Dimensional Printed Hydroxyapatite Bone Substitutes Designed by a Novel Periodic Minimal Surface Algorithm Are Highly Osteoconductive. 3D Printing and Additive Manufacturing, 10(5):905-916.
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Bioactive and Elastic Emulsion Electrospun DegraPol Tubes Delivering IGF-1 for Tendon Rupture Repair. International Journal of Molecular Sciences, 24(12):10272.
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Influence of Scaffold Microarchitecture on Angiogenesis and Regulation of Cell Differentiation during the Early Phase of Bone Healing: A Transcriptomics and Histological Analysis. International Journal of Molecular Sciences, 24(6):6000.
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3D Printing of Dental Prostheses: Current and Emerging Applications. Journal of Composites Science, 7(2):80.
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Osteoconductivity of bone substitutes with filament-based microarchitectures: Influence of directionality, filament dimension, and distance. International Journal of Bioprinting, 9(1):626.
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Osteoblasts. In: Gruber, Reinhard; Stadlinger, Bernd; Terheyden, Hendrik. Cell-Atlas – Visual Biology in Oral Medicine. Berlin: Quintessence Publishing, 135-144.
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Effect of N-Vinyl-2-Pyrrolidone (NVP), a Bromodomain-Binding Small Chemical, on Osteoblast and Osteoclast Differentiation and Its Potential Application for Bone Regeneration. International Journal of Molecular Sciences, 22(20):11052.
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Exposure to the bromodomain inhibitor N-methyl pyrrolidone blocks spermatogenesis in a hormonal and non-hormonal fashion. Toxicology and applied pharmacology, 423:115568.
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Therapeutic potential of mesenchymal stem cell-derived extracellular vesicles in regenerative endodontics. European Cells and Materials (ECM), 41:233-244.
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Microporosities in 3D-Printed Tricalcium-Phosphate-Based Bone Substitutes Enhance Osteoconduction and Affect Osteoclastic Resorption. International Journal of Molecular Sciences, 21(23):9270.
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Reversible Contraceptive Potential of FDA Approved Excipient N, N-Dimethylacetamide in Male Rats. Frontiers in Physiology, 11:601084.
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From Influenza Virus to Novel Corona Virus (SARS-CoV-2)–The Contribution of Obesity. Frontiers in Endocrinology, 11:556962.
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Transforming Growth Factor Beta 1 Distribution and Content in the Root Dentin of Young Mature and Immature Human Premolars. Journal of Endodontics, 46(5):641-647.
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Smart Hydrogels for the Augmentation of Bone Regeneration by Endogenous Mesenchymal Progenitor Cell Recruitment. Advanced Science, 7(7):1903395.
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Pulp-derived exosomes in a fibrin-based regenerative root filling material. Journal of Clinical Medicine, 9(2):491.
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Similar inductive effects of enamel and dentin matrix derivatives on osteoblast-like cell response over SLA titanium surface. Archives of Oral Biology, 109:104552.
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N,N-Dimethlyacetamide Prevents the High-Fat Diet-Induced Increase in Body Weight. Frontiers in Pharmacology, 10:1274.
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Reconsidering Osteoconduction in the Era of Additive Manufacturing. Tissue engineering. Part B, Reviews, 25(5):375-386.
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Osteoconductive Lattice Microarchitecture for Optimized Bone Regeneration. 3D Printing and Additive Manufacturing, 6(1):40-49.
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Biomimetic Conditioning of Human Dentin Using Citric Acid. Journal of Endodontics, 45(1):45-50.
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The Bromodomain Inhibitor N-Methyl pyrrolidone Prevents Osteoporosis and BMP-Triggered Sclerostin Expression in Osteocytes. International Journal of Molecular Sciences, 19(11):3332.
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Lattice Microarchitecture for Bone Tissue Engineering from Calcium Phosphate Compared to Titanium. Tissue Engineering. Part A, 24(19-20):1554-1561.
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Osteoconductive Microarchitecture of Bone Substitutes for Bone Regeneration Revisited. Frontiers in Physiology, 9:960.
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N,N Dimethylacetamide a drug excipient that acts as bromodomain ligand for osteoporosis treatment. Scientific Reports, 7:42108.
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Biodegradation and tissue integration of various polyethylene glycol matrices: a comparative study in rabbits. Clinical Oral Implants Research, 28(11):e244-e251.
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Role of HTRA1 in bone formation and regeneration: In vitro and in vivo evaluation. PLoS ONE, 12(7):e0181600.
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Iodixanol as a contrast agent in a fibrin hydrogel for endodontic applications. Frontiers in Physiology, 8:152.
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Influence of N-methyl pyrrolidone on the activity of the pulp-dentine complex and bone integrity during osteoporosis. International Endodontic Journal, 50(3):271-280.
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Heterodimeric BMP-2/7 for nucleus pulposus regeneration-In vitro and ex vivo studies. Journal of Orthopaedic Research, 35(1):51-60.
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Effects of stem cell factor on cell homing during functional pulp regeneration in human immature teeth. Tissue Engineering. Part A, 23(3-4):115-123.
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Comparative study of NMP-preloaded and dip-loaded membranes for guided bone regeneration of rabbit cranial defects. Journal of Tissue Engineering and Regenerative Medicine, 11(2):425-433.
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Epigenetic Regulation of Bone Remodeling and Its Impacts in Osteoporosis. International Journal of Molecular Sciences, 17(9):online.
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The Use of Adipose Tissue-Derived Progenitors in Bone Tissue Engineering - a Review. Transfusion Medicine and Hemotherapy, 43(5):336-343.
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Regenerative dentistry: animal model for regenerative endodontology. Transfusion Medicine and Hemotherapy, 43(5):359-364.
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Cell-Mediated Proteolytic Release of Growth Factors from Poly(Ethylene Glycol) Matrices. Macromolecular Bioscience, 16(11):1703-1713.
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Decreased fibrogenesis after treatment with pirfenidone in a newly developed mouse model of intestinal fibrosis. Inflammatory Bowel Diseases, 22(3):569-582.
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The bromodomain inhibitor N-methyl pyrrolidone reduced fat accumulation in an ovariectomized rat model. Clinical Epigenetics, 8:42.
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Influence of Microarchitecture on Osteoconduction and Mechanics of Porous Titanium Scaffolds Generated by Selective Laser Melting. 3D Printing and Additive Manufacturing, 3(3):142-151.
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Properties and Mechanobiological Behavior of Bovine Nasal Septum Cartilage. Annals of Biomedical Engineering, 44(5):1821-1831.
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N-methyl pyrrolidone (NMP) inhibits lipopolysaccharide-induced inflammation by suppressing NF-κB signaling. Inflammation Research, 64(7):527-536.
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Fibrin hydrogel based bone substitute tethered with BMP-2 and BMP-2/7 heterodimers. Materials, 8(3):977-991.
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BMP-2 and BMP-2/7 heterodimers conjugated to a fibrin/hyaluronic acid hydrogel in a large animal model of mild intervertebral disc degeneration. BioResearch Open Access, 4(1):398-406.
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Longitudinal in vivo evaluation of bone regeneration by combined measurement of multi-pinhole SPECT and micro-CT for tissue engineering. Scientific Reports, 5(10238):online.
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N-methyl pyrrolidone/bone morphogenetic protein-2 double delivery with in situ forming implants. Journal of Controlled Release, 203:181-188.
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Modular Poly(ethylene glycol) Matrices for the Controlled 3D-Localized Osteogenic Differentiation of Mesenchymal Stem Cells. Advanced Healthcare Materials, 4(4):550-558.
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Delivery of BMP-2 by two clinically available apatite materials: In vitro and in vivo comparison. Journal of Biomedical Materials Research. Part A, 103(2):628-638.
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Effect of platelet-derived growth factor-BB on tissue integration of cross-linked and non-cross-linked collagen matrices in a rat ectopic model. Clinical Oral Implants Research, 26(3):263-270.
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Fibrin gel improves tissue ingrowth and cell differentiation in human immature premolars implanted in rats. Journal of Endodontics, 40(2):246-250.
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A model system of the dynamic loading occurring in synovial joints: The biological effect of plowing on pristine cartilage. Cells, Tissues, Organs, 199(5-6):364-372.
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Effect of direct current on surface structure and cytocompatibility of titanium dental implants. International Journal of Oral & Maxillofacial Implants, 29(3):735-742.
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Effect of different rhBMP-2 and TG-VEGF ratios on the formation of heterotopic bone and neovessels. BioMed Research International, 2014:571510.
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Heterotopic bone formation around vessels: pilot study of a new animal model. BioResearch Open Access, 2(4):266-272.
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Immobilization of chondroitin sulfate to lipid membranes and its interactions with ECM proteins. Journal of Colloid and Interface Science, 390(1):258-266.
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Bone regeneration by the osteoconductivity of porous titanium implants manufactured by selective laser melting: A histological and micro computed tomography study in the rabbit. Tissue Engineering. Part A, 19(23-24):2645-2654.
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Evaluation of moldable, in situ hardening calcium phosphate bone graft substitutes. Clinical Oral Implants Research, 24(2):149-157.
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A versatile approach to engineering biomolecule-presenting cellular microenvironments. Advanced Healthcare Materials, 2(2):292-296.
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Intraoperative engineering of osteogenic grafts combining freshly harvested, human adipose-derived cells and physiological doses of bone morphogenetic protein-2. European Cells and Materials (ECM), 24:308-319.
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Effect of cerium chloride application on fibroblast and osteoblast proliferation and differentiation. Archives of Oral Biology, 57(7):892-897.
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Impact of recombinant platelet-derived growth factor BB on bone regeneration: a study in rabbits. International Journal of Periodontics and Restorative Dentistry, 32(2):195-202.
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Pretreatment thrombocytosis: a prognostic marker for oral squamous cell carcinoma?. Oral and Maxillofacial Surgery, 16(2):197-200.
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Biomaterial development for oral and maxillofacial bone regeneration. Journal of the Korean Association of Oral and Maxillofacial Surgeons, 38(5):264-270.
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Engineering 3D cell instructive microenvironments by rational assembly of artificial extracellular matrices and cell patterning. Integrative Biology, 3(11):1102-1111.
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Biodegradation, soft and hard tissue integration of various polyethylene glycol hydrogels: a histomorphometric study in rabbits. Clinical Oral Implants Research, 22(11):1247-1254.
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Coupling plowing of cartilage explants with gene expression in models for synovial joints. Journal of Biomechanics, 44(13):2472-2476.
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Inhibition of osteoclast differentiation and bone resorption by N-methylpyrrolidone. Journal of Biological Chemistry, 286(27):24458-24466.
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Design, construction and validation of a computer controlled system for functional loading of soft tissue. Medical Engineering and Physics, 33(6):377-683.
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Elucidating the role of matrix stiffness in 3D cell migration and remodeling. Biophysical Journal, 100(2):284-293.
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Guided bone regeneration with a synthetic biodegradable membrane: a comparative study in dogs. Clinical Oral Implants Research, 22(8):802-807.
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Bone regeneration using a synthetic matrix containing enamel matrix derivate. Clinical Oral Implants Research, 22(2):214-222.
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Bone regeneration in the presence of a synthetic hydroxyapatite/silica oxide-based and a xenogenic hydroxyapatite-based bone substitute materia. Clinical Oral Implants Research, 22(5):506-511.
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Enzyme mediated site-specific surface modification. Langmuir, 26(13):11127-11134.
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Mechanical loading of mouse caudal vertebrae increases trabecular and cortical bone mass-dependence on dose and genotype. Biomechanics and Modeling in Mechanobiology, 9(6):737-747.
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High power-pulsed Nd:YAG laser as a new stimulus to induce BMP-2 expression in MC3T3-E1 osteoblasts. Lasers in Surgery and Medicine, 42(6):510-518.
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Effects of muCT radiation on tissue engineered bone-like constructs. Biomedizinische Technik. Biomedical Engineering, 55(4):245-250.
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Enhanced osteoblastic activity and bone regeneration using surface-modified porous bioactive glass scaffolds. Journal of Biomedical Materials Research. Part A, 94(4):1023-1033.
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Biomimetic PEG hydrogels crosslinked with minimal plasmin-sensitive tri-amino acid peptides. Journal of Biomedical Materials Research. Part A, 93(3):870-877.
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Effect of short-time povidone-iodine application on osteoblast proliferation and differentiation. Open Dentistry Journal, 3:208-212.
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N-methyl pyrrolidone as a potent bone morphogenetic protein enhancer for bone tissue regeneration. Tissue Engineering. Part A, 15(10):2955-2963.
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Comparison of two resorbable membrane systems in bone regeneration after removal of wisdom teeth: a randomized-controlled clinical pilot study. Clinical Oral Implants Research, 20(10):1084-1091.
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cAMP enhances BMP2-signaling through PKA and MKP1-dependent mechanisms. Biochemical and Biophysical Research Communications (BBRC), 381(2):247-252.
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Bone morphogenetic protein-2 enhances bone formation when delivered by a synthetic matrix containing hydroxyapatite/tricalciumphosphate. Clinical Oral Implants Research, 19(2):188-195.
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The Zirconia implant-bone interface: a preliminary histologic evaluation in rabbits. International Journal of Oral and Maxillofacial Implants, 23(4):691-695.
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A novel, tissue occlusive poly(ethylene glycol) hydrogel material. Journal of Biomedical Materials Research Part A, 85A(2):285-292.
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Enzymatic formation of modular cell-instructive fibrin analogs for tissue engineering. Biomaterials, 28(26):3856-6386.
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Platelet-rich plasma and fibrin as delivery systems for recombinant human bone morphogenetic protein-2. Clinical Oral Implants Research, 16(6):676-682.
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Bone repair with a form of BMP-2 engineered for incorporation into fibrin cell ingrowth matrices.. Biotechnology and Bioengineering, 89(3):253-262.
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Enhancement of bone healing using non-glycosylated rhBMP-2 released from a fibrin matrix in dogs and cats.. Journal of Small Animal Practice, 46(1):17-21.
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Synthetic extracellular matrices for in situ tissue engineering.. Biotechnology and Bioengineering, 86(1):27-36.
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Bone healing in the rat and dog with nonglycosylated BMP-2 demonstrating low solubility in fibrin matrices.. Journal of Orthopaedic Research, 22(2):376-381.
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Treatment of nonunions with nonglycosylated recombinant human bone morphogenetic protein-2 delivered from a fibrin matrix.. Veterinary Surgery, 33(2):112-118.
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Deletion mutants of BMP folding variants act as BMP antagonists and are efficient inhibitors for heterotopic ossification.. Journal of Bone and Mineral Research, 18(12):2142-2151.
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Effect of rhBMP-2 on guided bone regeneration in humans. Clinical Oral Implants Research, 14(5):556-568.
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Slow and continuous application of human recombinant bone morphogenetic protein via biodegradable poly(lactide-co-glycolide) foamspheres.. International Journal of Oral and Maxillofacial Surgery, 31(1):60-65.
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Disulfide bridge conformers of mature BMP are inhibitors for heterotopic ossification.. Biochemical and Biophysical Research Communications (BBRC), 286(3):554-558.
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Knochenersatzmaterialien. Mund-, Kiefer- und Gesichtschirurgie, 4(S1):S384-S391.